Exploring the Underlying Mechanism of Alpinia officinarum Hance Ameliorating Diabetic Gastroparesis through Combining Network Pharmacology, Molecular Docking, and in Vivo Experimental Verification

Background: Alpinia officinarum Hance (AOH) has a long history in China as Chinese medicine and exerts the pharmacological effects of antidiabetic gastrointestinal protection. In traditional theory, AOH is often combined with other medicines for treatment diabetic gastroparesis (DGP). However, molecular mechanisms, potential targets, bioactive ingredients that act against DGP are yet to be elucidated. this study, network pharmacology, docking, experimental study were used predict therapeutic mechanism DGP. Methods: Network pharmacology analysis was performed acquire information on active chemical ingredients, DGP-related target proteins AOH, signaling pathway. addition, docking approach simulate binding drugs targets. Finally, DGP-mice model verification vivo. Results: Through research, AKT1 found core protein mainly involved PI3K-AKT Additionally, interactions between compounds (PIK3CA AKT1) analyzed using which verified results pharmacology. Further vivo studies indicated could reduce fasting blood glucose levels, improve gastric emptying rate, ameliorate biochemical indicators mice. Moreover, increase expressions phosphorylation levels PI3K AKT stomach regulate oxidative stress. Conclusions: The shown may play protective role through mediation pathway